Journal: Nature

Article Title: AIM2 in regulatory T cells restrains autoimmune diseases.

doi: 10.1038/s41586-021-03231-w

Figure Lengend Snippet: Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the RACK1–PP2A complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three

Article Snippet: Ectopic expression of PP2A and RACK1 in Treg cells To generate the retrovirus expressing PP2A and RACK1, we first cloned PP2A (OriGene Technologies, MR204384L4) and RACK1 (OriGene Technologies, MR204575L3) into retroviral vectors MSCV-IRES-Thy1.1 (MIT, Addgene 17442) and MSCV-IRES-GFP (MIG, Addgene 20672) respectively, and generated MIT-PP2A and MIG-RACK1 retrovirus in 293T cells by transient transfection.

Techniques: In Vitro, Reverse Transcription Polymerase Chain Reaction, Flow Cytometry, RNA Sequencing, Western Blot

Journal: eLife

Article Title: Natural Tr1-like cells do not confer long-term tolerogenic memory

doi: 10.7554/eLife.44821

Figure Lengend Snippet:

Article Snippet: Antibody , InVivoMAb mouse monoclonal anti-mouse Thy1.1/CD90.1 (clone 19E12) , BioXcell , Cat: BE0214 , 200 ug/mouse.

Techniques: Flow Cytometry

Journal:

Article Title: A cholera toxoid-insulin conjugate as an oral vaccine against spontaneous autoimmune diabetes

doi:

Figure Lengend Snippet: T cells from CTB-insulin-fed NOD mice suppress beta cell infiltration by diabetogenic T cells. Immunofluorescence staining of pancreatic islets from irradiated NOD males 30 days after co-injection of syngeneic Thy-1,2+ T cells from 20-week-old diabetic female mice with Thy-1,1+ T cells from congenic NOD mice that were fed CTB-insulin (A, C) or CTB-ovalbumin (B, D). Immunostainings were performed with monoclonal antibodies to Thy-1,2+ (A, B) and Thy-1,1+ (C, D). Original magnifications ×250.

Article Snippet: The percentage of donor Thy-1,1+ T cells in the spleen, mesenteric, and pancreatic lymph nodes of recipient NOD males was determined 7, 15, and 30 days after cell transfer by cytofluorometric analysis using FITC-labeled rat monoclonal antibodies to Thy-1,1 (clone CL005F; Cedarlane Laboratories) or Thy-1,2 (clone 30H12; Biosys, Compiègne, France) ( 32 ).

Techniques: Immunofluorescence, Staining, Irradiation, Injection

Journal: The American Journal of Pathology

Article Title: Identification and Characterization of Mesenchymal-Epithelial Progenitor-Like Cells in Normal and Injured Rat Liver

doi: 10.1016/j.ajpath.2014.08.029

Figure Lengend Snippet: Primary Antibodies Used in This Study

Article Snippet: Mouse anti-rat THY1/CD90 , IgG1 , Cedarlane (Hornby, ON, Canada) , CL005AP , 1:50.

Techniques: Transduction